We are dedicated to making a real difference in the lives of patients around the world. We realise that in order to accomplish this, we need talented, motivated individuals who believe in this mission and who seek excellence in their work. We are therefore committed to providing outstanding rewards for your contributions, a world-class working environment, and opportunities for career development.
Being consistently ranked among the world’s top cities for quality of life (No. 1 in 2009 and 2010), Vienna offers an attractive, varied and international environment.
Scientist in Phage Display
Posting date: 10 October 2011
We currently have an opening for a Scientist in phage display, who will focus on the selection, screening and optimization of antibodies against a variety of therapeutic targets. The position will be graded as Associate Scientist, Scientist or Senior Scientist depending on the experience of the successful candidate.
Responsibilities:
- Maintain and quality-control phage display libraries
- Devise selection strategies and design selection arms
- Biotinylate and quality-control antigens
- Perform phage selections using manual and automated techniques
- Perform high-throughput screening using ELISA automation
- Analyse high-throughput screening data
- Express selected antibody clones in E.coli
- Perform affinity maturation and optimization of antibodies
- Participate in the construction of antibody libraries
- Participate in the development of new antibody technology
Requirements:
- At least 3 years of hands-on research experience in protein and/or peptide phage display, having performed successful phage selection and screening campaigns on at least 2 targets
- Thorough understanding of the calculations required for phage library selections and of the mathematics that underlie the kinetics of protein-protein interaction
- Experience in the day-to-day cloning of cDNAs, protein expression in E.coli and basic protein chemistry such as affinity purification and Western Blotting
- Ability to accurately record and effectively communicate scientific data and ideas in English
- Commitment to keeping abreast of the latest literature in the field of phage display
Experience in affinity maturation and in the cloning of libraries would be of advantage.
If you believe that you are qualified for this position and this opportunity excites you, please submit your resume, including contact details for 2 referees, and cover letter to
jobs@dutalys.com